| 质粒名称: | pET28a-Beclin1 CC |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 24403 |
| 存储实验室: | Qing Zhong |
|
载体骨架基本信息 |
|
| 载体名称: | pET-28a |
| 空载体大小: | 5400bp |
| 完整质粒大小: | 5400 bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | Kanamycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Beclin1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 273bp |
| 基因突变: | Contains the CC domain (aa 151-242) |
| 标签/融合蛋白: | His (N terminal on backbone) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI (unknown if destroyed) |
| 3’克隆位点: | NotI (unknown if destroyed) |
| 5’测序引物 | T7 |
|
文献引用方法 |
|
| 材料和方法部分: |
pET28a-Beclin1 CC was a gift from Qing Zhong (Addgene plasmid # 24403 ; http://n2t.net/addgene:24403 ; RRID:Addgene_24403)
|
| 参考文献部分: | Identification of Barkor as a mammalian autophagy-specific factor for Beclin 1 and class III phosphatidylinositol 3-kinase. Sun Q, Fan W, Chen K, Ding X, Chen S, Zhong Q. Proc Natl Acad Sci U S A. 2008 Dec 9. 105(49):19211-6. 10.1073/pnas.0810452105 PubMed 19050071 |
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